Outbreak of Stenotrophomonas maltophilia and Burkholderia cepacia Bloodstream Infections at a Hemodialysis Center.

Patients undergoing hemodialysis are at an increased risk for bloodstream infections (BSIs). Infection usually occurs because of contamination of water supply, water treatment, distribution systems, or reprocessing dialyzers. Here, we report an outbreak of BSIs caused by Stenotrophomonas maltophilia (n = 21) and Burkholderia cepacia (n = 22) among dialyzed patients at a large hemodialysis center in Brazil. Overall, three patients died (7%), two of which had bacteremia caused by S. maltophilia and the other had a B. cepacia infection. We collected water samples from different points of the hemodialysis system for culture and typing. Genetic patterns were identified through polymerase chain reaction-random amplified polymorphic DNA (PCR-RAPD) and pulsed-field gel electrophoresis. The same genotypes of S. maltophilia and B. cepacia recovered from blood cultures were found in dialysis water. Also, multiple genetic profiles were identified among water isolates, suggesting heavy contamination. Bacteremia cases persisted even after implementing standard control measures, which led us to believe that the piping system was contaminated with microbial biofilms. Soon after we changed the entire plumbing system, reported cases dropped back to the number typically expected, and the outbreak came to an end.

Effects caused by the spinal administration of ketamine S (+) 5% with no preservatives, using a single puncture, and located on the spinal cord and meninges in rabbits.

PURPOSE: To evaluate the effect of ketamine S (+) 5% with no preservatives and administered as a subarachnoid single puncture on the spinal cord and meninges of rabbits. METHODS: Twenty young adult female rabbits, each weighing 3500-5000 g and having a spine length between 34 and 38 cm, were divided by lot into two groups (G): 0.9% saline in G1 and ketamine S (+) 5% in G2, by volume of 5 µg per cm column (0.18 mL). After intravenous anaesthesia with ketamine and xylazine, the subarachnoid space was punctured at S1-S2 under ultrasound guidance, and a random solution was injected. The animals remained in captivity for 21 days under medical observation and were sacrificed by decapitation. The lumbosacral spinal cord portion was removed for immunohistochemistry to assess the glial fibrillary acidic protein (GFAP), and histology was assessed using hematoxylin and eosin (HE) stain. RESULTS: No histological lesions were found in the nervous tissue (roots and cord) or meninges in either group. CONCLUSION: The ketamine S (+) 5% unpreserved triggered no neurological or histological lesions in the spinal cord or meninges of rabbits.

Effects caused by the spinal administration of ketamine S (+) 5% with no preservatives, using a single puncture, and located on the spinal cord and meninges in rabbits

PURPOSE: To evaluate the effect of ketamine S (+) 5% with no preservatives and administered as a subarachnoid single puncture on the spinal cord and meninges of rabbits. METHODS: Twenty young adult female rabbits, each weighing 3500-5000 g and having a spine length between 34 and 38 cm, were divided by lot into two groups (G): 0.9% saline in G1 and ketamine S (+) 5% in G2, by volume of 5 μg per cm column (0.18 mL). After intravenous anaesthesia with ketamine and xylazine, the subarachnoid space was punctured at S1-S2 under ultrasound guidance, and a random solution was injected. The animals remained in captivity for 21 days under medical observation and were sacrificed by decapitation. The lumbosacral spinal cord portion was removed for immunohistochemistry to assess the glial fibrillary acidic protein (GFAP), and histology was assessed using hematoxylin and eosin (HE) stain. RESULTS: No histological lesions were found in the nervous tissue (roots and cord) or meninges in either group. CONCLUSION: The ketamine S (+) 5% unpreserved triggered no neurological or histological lesions in the spinal cord or meninges of rabbits. .

Reevaluation of the airways of obese patients undergone bariatric surgery after reduction in body mass index.

BACKGROUND AND OBJECTIVES: Difficulty intubating is a cause of mortality in anesthesiology and it can be related to obesity. The diagnosis of difficult intubation contributes for successful airways management. However, parameters that predict difficult airways are not well established. Mallampati classification, the interincisor gap, neck circumference, thyromental distance, and the presence of obstructive sleep apnea, are parameters that can indicate difficult intubation. Surgical treatment of obesity provides reduction in body mass index (BMI) with stabilization after about 2 years. The objective of the present study was to reevaluate the parameters described above and compare them with pre-surgical values. METHODS: The BMI, Mallampati classification, neck circumference, interincisor gap, thyromental distance, and the degree of obstructive sleep apnea in polysomnography were evaluated in 51 patients of both genders in the preoperative period. Two years after the surgery and reduction of the BMI to < 35 kg.m(-2), predictor factors of difficult airways were reevaluated by another anesthesiologist who knew the patients' BMI before surgery. Nine patients were excluded. The new reevaluation was performed, and for those who did not have another polysomnography the somnolence scale of Epiworth was applied. RESULTS: Forty-two patients were reevaluated. They showed a reduction in BMI and neck circumference, and an increase in both interincisor gap and thyromental distance. Only one patient showed a reduction in Mallampati scale, and only 4 patients performed polysomnography. CONCLUSIONS: Reduction of the BMI allows for an increased interincisor gap, thyromental distance, and reduction in neck circumference. Mallampati classification remains the same.

Avaliação da função pulmonar em pacientes submetidas à mastoplastia redutora / Evaluation of pulmonary function in patients submitted to reduction mammaplasty

OBJETIVO: Avaliar prospectivamente a função pulmonar de pacientes submetidas à mastoplastia redutora. MÉTODOS: Doze pacientes femininas portadoras de gigantomastia e sem antecedentes médicos, realizaram mastoplastia redutora no Hospital das Clínicas da UFBA. As pacientes foram submetidas ao teste de função pulmonar e medidas de gases sanguíneos arteriais nos períodos pré-operatório e pós-operatório (três a seis meses). Os dados obtidos foram analisados por meio do teste de Wilcoxon e o nível de significância estatística foi p< 0,05. RESULTADOS: Nos dados obtidos por intermédio dos testes de função pulmonar, a capacidade pulmonar total e o volume residual foram maiores no pós-operatório (p < 0,05). Quanto aos dados de gases arteriais, não houve variações estatisticamente significantes. CONCLUSÃO: O aumento da capacidade pulmonar total e volume residual podem sugerir uma melhor função pulmonar após mastoplastia redutora em gigantomastia, apesar de não alterarem os gases arteriais das pacientes saudáveis.

OBJECTIVE: To prospectively evaluate the pulmonary function in patients undergoing reduction mammaplasty. METHODS: Twelve female patients with Gigantomastia and without medical antecedents were submitted to reduction mammaplasty at our University Hospital. The patients underwent lung function testing and arterial blood gas measurements in the preoperative and postoperative period (three to six months). The data were analyzed using the Wilcoxon test and the level of statistical significance was p <0.05. As for lung function tests,total lung capacity and residual volume were higher postoperatively (p <0.05). RESULTS: As for the arterial gases, there was no statistically significant difference. CONCLUSION: The increase in total lung capacity and residual volume may suggest a better lung function after reduction mammaplasty for Gigantomastia treatment, although not altering blood gases in healthy patients.

Reavaliação da via aérea do paciente obeso submetido à cirurgia bariátrica após a redução do índice de massa corpórea / Reevaluation of the airways of obese patients undergone bariatric surgery after reduction in body mass index / Nueva evaluación de la vía aérea del paciente obeso sometido a la cirugía bariátrica, después de la reducción del índice de masa corporal

JUSTIFICATIVA E OBJETIVOS: A dificuldade na intubação traqueal é causa de mortalidade em anestesiologia e pode estar relacionada à obesidade. Diagnosticar uma intubação difícil contribui para o êxito da abordagem da via aérea, mas os parâmetros preditores de intubação difícil não estão bem estabelecidos. A classificação de Mallampati, a distância interincisivos, a circunferência do pescoço, a distância tireomentoniana e a presença da síndrome da apneia obstrutiva do sono são parâmetros que podem indicar uma intubação difícil. O tratamento cirúrgico da obesidade proporciona redução do índice de massa corpórea (IMC), com estabilização por volta de 2 anos. O objetivo desta pesquisa foi reavaliar os parâmetros descritos com os valores pré-cirúrgicos. MÉTODO: Cinquenta e um pacientes de ambos os sexos foram avaliados no período pré-operatório quanto a IMC, classificação de Mallampati, circunferência do pescoço, distância interincisivos, distância tireomentoniana e grau da síndrome de apneia obstrutiva do sono por meio da polissonografia. Após dois anos de cirurgia e redução do IMC < 35 kg.m-2, os preditores de intubação difícil foram reavaliados por outro médico anestesiologista com conhecimento do IMC prévio. Foram excluídos nove pacientes. Executou-se reavaliação dos parâmetros supracitados e, para os que não realizaram nova polissonografia, aplicou-se a escala de sonolência de Epiworth. RESULTADOS: Quarenta e dois pacientes foram reavaliados e mostraram redução do IMC e da circunferência do pescoço e aumento das distâncias interincisivos e tireomentoniana. Apenas um paciente apresentou redução na escala de Mallampati e somente quatro realizaram a polissonografia. CONCLUSÕES: A redução do IMC proporciona aumento das distâncias interincisivos e tireomentoniana e redução da circunferência do pescoço. O Mallampati permanece inalterado.

BACKGROUND AND OBJECTIVES: Difficulty intubating is a cause of mortality in anesthesiology and it can be related to obesity. The diagnosis of difficult intubation contributes for successful airways management. However, parameters that predict difficult airways are not well established. Mallampati classification, the interincisor gap, neck circumference, thyromental distance, and the presence of obstructive sleep apnea, are parameters that can indicate difficult intubation. Surgical treatment of obesity provides reduction in body mass index (BMI) with stabilization after about 2 years. The objective of the present study was to reevaluate the parameters described above and compare them with pre-surgical values. METHODS: The BMI, Mallampati classification, neck circumference, interincisor gap, thyromental distance, and the degree of obstructive sleep apnea in polysomnography were evaluated in 51 patients of both genders in the preoperative period. Two years after the surgery and reduction of the BMI to < 35 kg.m-2, predictor factors of difficult airways were reevaluated by another anesthesiologist who knew the patients' BMI before surgery. Nine patients were excluded. The new reevaluation was performed, and for those who did not have another polysomnography the somnolence scale of Epiworth was applied. RESULTS: Forty-two patients were reevaluated. They showed a reduction in BMI and neck circumference, and an increase in both interincisor gap and thyromental distance. Only one patient showed a reduction in Mallampati scale, and only 4 patients performed polysomnography. CONCLUSIONS: Reduction of the BMI allows for an increased interincisor gap, thyromental distance, and reduction in neck circumference. Mallampati classification remains the same.

JUSTIFICATIVA Y OBJETIVOS: La dificultad en la intubación traqueal es la causa de mortalidad en anestesiología y puede estar relacionada con la obesidad. Diagnosticar la intubación difícil contribuye para el éxito del abordaje de la vía aérea, pero los parámetros de predicción de la intubación difícil todavía no están bien establecidos. La clasificación de Mallampati, la distancia interincisivos, la circunferencia del cuello, la distancia tiromentoniana y la presencia del síndrome de la apnea obstructiva del sueño son parámetros que pueden indicar la intubación difícil. El tratamiento quirúrgico de la obesidad proporciona una reducción del índice de masa corporal (IMC), con su estabilización alrededor de los dos años. El objetivo de esta investigación fue evaluar nuevamente los parámetros anteriormente descritos con los valores prequirúrgicos. MÉTODO: Cincuenta y un pacientes de los dos sexos, fueron evaluados en el período preoperatorio en cuanto a su IMC, la clasificación de Mallampati, la circunferencia del cuello, la distancia interincisivos, la distancia tiromentoniana y el grado del síndrome de la apnea obstructiva del sueño, por medio de la polisonografía. Después de dos años de efectuada la cirugía y la reducción del IMC < a35 kg.m-2 los predictores de intubación difícil se evaluaron nuevamente por otro médico anestesiólogo que conocía el IMC con anterioridad. Fueron excluidos nueve pacientes. Se ejecutó la nueva evaluación de los parámetros anteriormente citados, y para los que no se realizó una nueva polisonografía se aplicó la escala de somnolencia de Epiworth. RESULTADOS: De los 42 pacientes evaluados de nuevo, algunos presentaron reducción del IMC, de la circunferencia del cuello, de la distancia interincisivos y tiromentoniana. Apenas un paciente presentó reducción en la escala de Mallampati y solo 4 realizaron la polisonografía. CONCLUSIONES: La reducción del IMC aumenta las distancias interincisivas y tiromentonianas. Se verifica la reducción de la circunferencia del cuello. El Mallampati permanece sin alteraciones.